Journal: The Plant journal : for cell and molecular biology

Article Title: Alteration of cell wall xylan acetylation trigger defense responses that counterbalance the immune deficiencies of plants impaired in the β subunit of the heterotrimeric G protein

doi: 10.1111/tpj.13660

Figure Lengend Snippet: (a) Gene expression was determined by qRT–PCR in tissues from 21 day-old untreated plants and are represented as the average (n=3 ± SE). Transcript levels of analyzed genes were normalized to the Arabidopsis UBC21 gene. Asterisks indicate significant differences with Col-0 plants (Student's t-test, p < 0.05). This is a representative experiment of the three performed that gave similar results. (b) Comparative metabolite enrichment of esk1-7 and irx1-6 plants. The Venn diagram shows the number of miss-regulated metabolites identified in esk1-7 and irx1-6 in comparison to wild-type plants (p < 0.1). (c) Metabolic pathway enrichment in esk1-7 and irx1-6. Values were calculated as the number of experimentally regulated compounds (p < 0.05) relative to all detected compounds in a pathway, compared to the total number of experimentally regulated compounds relative to all detected compounds in the study (321). Pathways with less than three metabolites were not included in the analysis.

Article Snippet: Four biological replicates for each of these genotypes were further processed and analyzed by Metabolon Inc. (Research Triangle Park, North Carolina) for global unbiased metabolite profiling as described ( Ren et al. , 2012 ).

Techniques: Gene Expression, Quantitative RT-PCR, Comparison